Inheritance Cube: tGD

The trans-complementing Gene Drive (tGD) is a 1-locus, 2 target site drive. The first target site corresponds to the Cas protein, the second to an effector gene and the gRNAs. There are two sets of gRNAs, because each target site may have different cutting/homing/resistance rates, and each sex can have different rates for all of those things. Additionally, the parent that you receive your Cas from dictates its efficiency. Therefor, this construct has 5 alleles at the first locus and 4 alleles at the second.

  • Locus 1

    • W: Wild-type

    • P: Paternal Cas9

    • M: Maternal Cas9

    • R: Resistant allele 1

    • B: Resistant allele 2

  • Locus 2

    • W: Wild-type

    • G: gRNAs

    • R: Resistant 1

    • B: Resistant 2

cubeTGD(
  cM1 = 0,
  cM2 = 0,
  cP1 = 0,
  cP2 = 0,
  hM1 = 0,
  hM2 = 0,
  hP1 = 0,
  hP2 = 0,
  rM1 = 0,
  rM2 = 0,
  rP1 = 0,
  rP2 = 0,
  crM = 0,
  crP = 0,
  eta = NULL,
  phi = NULL,
  omega = NULL,
  xiF = NULL,
  xiM = NULL,
  s = NULL
)

Arguments

cM1

Maternally inherited Cas9 cutting rate at locus 1
cM2

Maternally inherited Cas9 cutting rate at locus 2
cP1

Paternally inherited Cas9 cutting rate at locus 1
cP2

Paternally inherited Cas9 cutting rate at locus 2
hM1

Maternally inherited Cas9 homing efficiency at locus 1
hM2

Maternally inherited Cas9 homing efficiency at locus 2
hP1

Paternally inherited Cas9 homing efficiency at locus 1
hP2

Paternally inherited Cas9 homing efficiency at locus 2
rM1

Maternally inherited Cas9 resistance efficiency at locus 1
rM2

Maternally inherited Cas9 resistance efficiency at locus 2
rP1

Paternally inherited Cas9 resistance efficiency at locus 1
rP2

Paternally inherited Cas9 resistance efficiency at locus 2
crM

Maternal crossover rate, 0 is completely linked, 0.5 is unlinked, 1.0 is complete divergence
crP

Paternal crossover rate, 0 is completely linked, 0.5 is unlinked, 1.0 is complete divergence
eta

Genotype-specific mating fitness
phi

Genotype-specific sex ratio at emergence
omega

Genotype-specific multiplicative modifier of adult mortality
xiF

Genotype-specific female pupatory success
xiM

Genotype-specific male pupatory success
s

Genotype-specific fractional reduction(increase) in fertility

Value

Named list containing the inheritance cube, transition matrix, genotypes, wild-type allele, and all genotype-specific parameters.

Details

This drive corresponds to the transcomplementing gene drive developed by the Gantz and Bier lab.