Inheritance Cube: Y-linked Split CRISPR Drive with 2 Resistance Alleles

This is a Y-linked version of a split CRISPR drive. At the Y-locus is the Cas9, inherited in a Mendelian fashion. At a second, unlinked, autosomal locus are the gRNAs. When the two loci occur together (i.e. in males), the gRNAs drive, with potential damaged alleles, but the Cas9 remains Mendelian. This drive has 2 loci:

• "Locus" 1, sex chromosomes, has 3 alleles:

  • X: Wild-type X chromosome

  • Y: Wild-type Y chromosome

  • C: Y chromosome with Cas9

• Locus 2, autosomal locus, has 4 alleles:

  • W: Wild-type allele

  • G: gRNA allele

  • R: Functional or low-cost resistance allele

  • B: Non-functional or high-cost resistance allele

Usage

cubeSplitDriveY(
  cM = 1,
  chM = 0,
  crM = 0,
  eta = NULL,
  phi = NULL,
  omega = NULL,
  xiF = NULL,
  xiM = NULL,
  s = NULL
)

Arguments

cM

Cutting efficiency in males

chM

Homing efficiency in males

crM

Resistance efficiency in males

eta

Genotype-specific mating fitness

phi

Genotype-specific sex ratio at emergence

omega

Genotype-specific multiplicative modifier of adult mortality

xiF

Genotype-specific female pupatory success

xiM

Genotype-specific male pupatory success

s

Genotype-specific fractional reduction(increase) in fertility

Value

Named list containing the inheritance cube, transition matrix, genotypes, wild-type allele, and all genotype-specific parameters.