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Inheritance Cube: X-linked Split CRISPR Drive with 2 Resistance Alleles and male/female specific homing

Source: R/Cube-SplitDriveX.R
cubeSplitDriveX.Rd

This is a X-linked, sex-specific version of a split CRISPR drive. At the X locus is the Cas9, inherited in a Mendelian fashion. At a second, unlinked, autosomal locus are the gRNAs. When the two loci occur together, the gRNAs drive, with potential damaged alleles, but the Cas9 remains Mendelian. Deposition in this cube is performed when both pieces come together in females. This drive has 2 loci:

  • "Locus" 1, sex chromosomes, has 3 alleles

    • X: Wild-type X chromosome

    • C: X-chromosome carrying a Cas9 construct

    • Y: Wild-type Y chromosome

  • Locus 2, autosomal locus, has 4 alleles:

    • W: Wild-type allele

    • G: gRNA allele

    • R: Functional or low-cost resistance allele

    • B: Non-functional or high-cost resistance allele

Usage

cubeSplitDriveX(
  cM = 1,
  chM = 0,
  crM = 0,
  cF = 1,
  chF = 0,
  crF = 0,
  ccF = cF,
  cchF = chF,
  ccrF = crF,
  dW = 0,
  dhW = 0,
  drW = 0,
  ddW = dW,
  ddhW = dhW,
  ddrW = drW,
  eta = NULL,
  phi = NULL,
  omega = NULL,
  xiF = NULL,
  xiM = NULL,
  s = NULL
)

Arguments

cM

Cutting efficiency in males

chM

Homing efficiency in males

crM

Resistance efficiency in males

cF

Cutting efficiency in females, one Cas9 allele

chF

Homing efficiency in females, one Cas9 allele

crF

Resistance efficiency in females, one Cas9 allele

ccF

Cutting efficiency in females, two Cas9 alleles

cchF

Homing efficiency in females, two Cas9 alleles

ccrF

Resistance efficiency in females, two Cas9 alleles

dW

Maternal deposition cutting, one Cas9 allele

dhW

Maternal deposition homing, one Cas9 allele

drW

Maternal deposition resistance, one Cas9 allele

ddW

Maternal deposition cutting, two Cas9 alleles

ddhW

Maternal deposition homing, two Cas9 alleles

ddrW

Maternal deposition resistance, two Cas9 alleles

eta

Genotype-specific mating fitness

phi

Genotype-specific sex ratio at emergence

omega

Genotype-specific multiplicative modifier of adult mortality

xiF

Genotype-specific female pupatory success

xiM

Genotype-specific male pupatory success

s

Genotype-specific fractional reduction(increase) in fertility

Value

Named list containing the inheritance cube, transition matrix, genotypes, wild-type allele, and all genotype-specific parameters.