Inheritance Cube: ClvR (Cleave and Rescue)

Based on the Cleave-and-Rescue system of Oberhofer, this is a 2-locus Cas9-based toxin-antidote system. The first locus carries the Cas9, gRNAs, and a recoded copy of an essential gene. The second locus is the targeted essential gene. This gene can be completely haplosufficient (hSuf = 1) or completely haploinsufficient (hSuf = 0). It is assumed that having 2 copies of the gene (be it wild-type at the second locus or recoded at the first) confers complete viability.

cubeClvR(
  cF = 1,
  crF = 0,
  ccF = cF,
  ccrF = crF,
  cM = 1,
  crM = 0,
  ccM = cM,
  ccrM = crM,
  dW = 0,
  drW = 0,
  ddW = dW,
  ddrW = drW,
  hSuf = 1,
  eta = NULL,
  phi = NULL,
  omega = NULL,
  xiF = NULL,
  xiM = NULL,
  s = NULL
)

Arguments

cF	Female cutting rate, one ClvR allele
crF	Female functional resistance rate, one ClvR allele
ccF	Female cutting rate, two ClvR alleles
ccrF	Female functional resistance rate, two ClvR alleles
cM	Male cutting rate, one ClvR allele
crM	Male functional resistance rate, one ClvR allele
ccM	Male cutting rate, two ClvR alleles
ccrM	Male functional resistance rate, two ClvR alleles
dW	Female deposition cutting rate
drW	Female deposition functional resistance rate
ddW	Female deposition (HH) cutting rate
ddrW	Female deposition (HH) functional resistance rate
hSuf	Haplosufficiency level, default is completely sufficient
eta	Genotype-specific mating fitness
phi	Genotype-specific sex ratio at emergence
omega	Genotype-specific multiplicative modifier of adult mortality
xiF	Genotype-specific female pupatory success
xiM	Genotype-specific male pupatory success
s	Genotype-specific fractional reduction(increase) in fertility

Value

Named list containing the inheritance cube, transition matrix, genotypes, wild-type allele, and all genotype-specific parameters.