Based on the Cleave-and-Rescue system of Oberhofer et. al. (doi:10.1073/pnas.1921698117
),
this is a 2-locus Cas9-based toxin-antidote system. The first locus carries the
Cas9, gRNAs, and a recoded copy of an essential gene. The second locus is the
targeted essential gene. This gene can be completely haplosufficient (hSuf = 1)
or completely haploinsufficient (hSuf = 0). It is assumed that having
2 copies of the gene (be it wild-type at the second locus or recoded at the first)
confers complete viability.
Usage
cubeClvR(
cF = 1,
crF = 0,
ccF = cF,
ccrF = crF,
cM = 1,
crM = 0,
ccM = cM,
ccrM = crM,
dW = 0,
drW = 0,
ddW = dW,
ddrW = drW,
hSuf = 1,
eta = NULL,
phi = NULL,
omega = NULL,
xiF = NULL,
xiM = NULL,
s = NULL
)Arguments
- cF
Female cutting rate, one ClvR allele
- crF
Female functional resistance rate, one ClvR allele
- ccF
Female cutting rate, two ClvR alleles
- ccrF
Female functional resistance rate, two ClvR alleles
- cM
Male cutting rate, one ClvR allele
- crM
Male functional resistance rate, one ClvR allele
- ccM
Male cutting rate, two ClvR alleles
- ccrM
Male functional resistance rate, two ClvR alleles
- dW
Female deposition cutting rate
- drW
Female deposition functional resistance rate
- ddW
Female deposition (HH) cutting rate
- ddrW
Female deposition (HH) functional resistance rate
- hSuf
Haplosufficiency level, default is completely sufficient
- eta
Genotype-specific mating fitness
- phi
Genotype-specific sex ratio at emergence
- omega
Genotype-specific multiplicative modifier of adult mortality
- xiF
Genotype-specific female pupatory success
- xiM
Genotype-specific male pupatory success
- s
Genotype-specific fractional reduction(increase) in fertility