This is a novel cube from the Akbari lab. There are 2 loci: the first is a female fertility locus (e.g. doubleSex), where the Cas, gRNAs, and a recoded essential gene go. This locus is inherited in a Mendelian fashion, but is also targeted for destruction by the homing allele. The second locus involves an essential gene, for both males and females, and this is the target of the gRNAs at the first locus. No homing is performed, it is simply destroyed. There is different cutting rates in males and females, with no possibility for a rescuing resistant allele. Females homozygous for the H or B alleles at locus 1 are viable but infertile, while males are unaffected. All animals homozygous at locus two must contain the recoded copy at locus 1 to be viable. This corresponds to the homing construct being X-linked.
Usage
cubeCLEAVRX(
cM1 = 1,
cM2 = 1,
cF1 = 1,
cF2 = 1,
eta = NULL,
phi = NULL,
omega = NULL,
xiF = NULL,
xiM = NULL,
s = NULL
)Arguments
- cM1
Male cutting rate at first locus IS NOT USED IN THIS CUBE
- cM2
Male cutting rate at second locus
- cF1
Female cutting rate at first locus
- cF2
Female cutting rate at second locus
- eta
Genotype-specific mating fitness
- phi
Genotype-specific sex ratio at emergence
- omega
Genotype-specific multiplicative modifier of adult mortality
- xiF
Genotype-specific female pupatory success
- xiM
Genotype-specific male pupatory success
- s
Genotype-specific fractional reduction(increase) in fertility