This is a modified split-drive construct for split-suppression drives. This is an autosomal, 2-locus drive. The first locus contains the Cas9 allele and the second locus has the gRNA construct. Cas9 efficacy in each sex is dependent upon parent of inheritance. This construct has 3 alleles at the first locus and 4 alleles at the second.
Locus 1
W: Wild-type
P: Paternal Cas9
M: Maternal Cas9
Locus 2
W: Wild-type
G: gRNAs
R: Resistant 1
B: Resistant 2
Usage
cubeASmidler(
cMM = 0,
chMM = 0,
crMM = 0,
cPM = 0,
chPM = 0,
crPM = 0,
cMF = 0,
chMF = 0,
crMF = 0,
cPF = 0,
chPF = 0,
crPF = 0,
crF = 0.5,
crM = 0.5,
eta = NULL,
phi = NULL,
omega = NULL,
xiF = NULL,
xiM = NULL,
s = NULL
)Arguments
- cMM
Cutting efficacy of maternally-inherited Cas9 in males
- chMM
Homing efficacy of maternally-inherited Cas9 in males
- crMM
Resistance rate of maternally-inherited Cas9 in males
- cPM
Cutting efficacy of paternally-inherited Cas9 in males
- chPM
Homing efficacy of paternally-inherited Cas9 in males
- crPM
Resistance rate of paternally-inherited Cas9 in males
- cMF
Cutting efficacy of maternally-inherited Cas9 in females
- chMF
Homing efficacy of maternally-inherited Cas9 in females
- crMF
Resistance rate of maternally-inherited Cas9 in females
- cPF
Cutting efficacy of paternally-inherited Cas9 in females
- chPF
Homing efficacy of paternally-inherited Cas9 in females
- crPF
Resistance rate of paternally-inherited Cas9 in females
- crF
Female crossover rate, 0 is completely linked, 0.5 is unlinked, 1.0 is complete divergence
- crM
Male crossover rate, 0 is completely linked, 0.5 is unlinked, 1.0 is complete divergence
- eta
Genotype-specific mating fitness
- phi
Genotype-specific sex ratio at emergence
- omega
Genotype-specific multiplicative modifier of adult mortality
- xiF
Genotype-specific female pupatory success
- xiM
Genotype-specific male pupatory success
- s
Genotype-specific fractional reduction(increase) in fertility